THE DEMOLITION OF IPATIEV HOUSE

 

DNA, LUMINOL AND THE DEMOLITION OF IPATIEV HOUSE

DO YOU STILL THINK THAT THERE WAS AN EXECUSION?

Romanov Family, Romanov Dynasty, is more than a memory, it is a permanent reality that has its own heartbeat within WORLD HISTORY AND RUSSIAN HISTORY.

A mystery? an execution? a false execution? data impossible to corroborate ? because in addition to a loose story of the Bolsheviks, of more than 100 years, there are interests not to arrive at the truth of the FORCED DISAPPEARANCE OF AN ENTIRE FAMILY FROM ONE DAY TO THE OTHER.

The IPATIEV house was demolished in 1977 on orders from the Politburo of the Communist Party of the Soviet Union to the local Soviet communist government, almost 59 years after the execution of the Romanov family and 14 years before the dissolution of the Soviet Union itself in 1990-1991 .NO ANTENTION CALLS YOU?

Diran became DNA, but they were also questioned.

Who benefited in the past is a subject of history and historical revisionism. The question is who continues to benefit today from lying?

We know that, for all kinds of scientific investigation, evidence and taking evidence are needed for forensic analysis. The primary, criminal scene, Ipatiev house was demolished; Sufficient evidence would have been found there to determine whether or not there was a bloody execution.

The first chemical reagents to blood appear in 1928.

*** When luminol is sprayed evenly on a surface, very small amounts of an oxidizing agent can activate it and cause it to emit a blue light, visible in a dark place. The glow lasts for about 30 seconds, it is possible to document the effect with a long exposure photograph. ***

In 1928, the German chemist H. O. Albrecht discovered that blood, among other substances, enhanced the luminescence of luminol in a hydrogen peroxide solution. In 1936, Karl Gleu and Karl Pfanstiel confirmed that this improvement was due to the presence of the heme group, a component of blood. In 1937, the German forensic scientist Walter Specht carried out extensive studies on the application of luminol to the detection of blood at crime scenes. In 1939, San Francisco pathologists Frederick Proescher and A. M. Moody made three important observations about this substance:

Although the result is based on the assumption that it is blood, large areas of suspicious material can be quickly examined.

Dry and degraded blood reacts more intensely and lastingly than fresh blood.

If the luminescence disappears, it can be reproduced again by adding a new luminol-hydrogen peroxide solution. Each spot of dried blood can be made to shine several times.

However, there are a number of drawbacks that can limit its usefulness in an investigation:

The chemiluminescence of luminol can also be triggered by the presence of substances such as copper or compounds that contain it, in addition to certain bleaching products. As a result, if someone thoroughly cleans a crime scene with a bleach product, the residue can cause the entire room to glow evenly, hiding any blood residue.

Radish-derived food products, through the enzyme horseradish peroxidase, catalyze the oxidation of luminol, emitting light at 428 nm (blue in the visible spectrum), which can result in a false positive.

Luminol can detect small amounts of blood present in urine. If there is animal urine on the treated surface, the result may be distorted.

It also reacts with fecal matter, causing the same glow as if it were blood.

The presence of lumionol can make other tests difficult. However, it has been shown that DNA can be extracted from luminol-treated samples.

Residual smoke particles (for example, in rooms where people smoke regularly) can cause a false positive.

DNA is known as the molecule of heredity and contains the information necessary for the generation of all eukaryotic organisms. Its discovery, studies and applications resulted in the leap into a new era, the era of DNA or Genomics. The meaning of its acronym reveals its molecular composition, DeoxyriboNucleic Acid, and its double helix structure is becoming more widely known every day.

DNA was first isolated by a Swiss biologist named Frierich Miescher in 1869. This scientist who studied the chemical composition of leukocytes (white blood cells), described from his experiments that the properties of the isolated substance rich in phosphates, without sulfur and protease resistant did not correspond to lipids or proteins. Miescher called this new molecule, present in all cell nuclei, nuclein. Then, with the identification of its acidic nature, it was assigned the generic name of nucleic acid.

In the 1920s, Phoebus Levene, in his studies of the structure and function of nucleic acids, managed to determine the existence of DNA and RNA, in addition to the fact that DNA is made up of 4 nitrogenous bases Thymine and Cytosine (pyrimidines), Guanine and Adenine (purines), a sugar (deoxyribose), and a phosphate group. He determined that the basic unit of DNA was made up of phosphate-sugar-nitrogen base, which he called nucleotide.

DNA

Then with the contributions of Griffith in 1928, the findings of Avery in 1944 and the Hershey-Chase experiments in 1952, it was possible to determine that DNA is the molecule responsible for inheritance. A year later Rosalind Franklin and Maurice Wilkins, Francis Crick and James Watson managed to elucidate by means of X-ray diffraction studies, the double helix molecular structure of DNA, which earned them the Novel Prize in Physiology and Medicine in 1962.

Already in the 21st century, advances in DNA technology, specifically in sequencing methods, have led to the knowledge of all the genetic information of a variety of organisms, such as human, mouse, zebrafish and A. thaliana, making possible enormous advances in disciplines as diverse as biomedicine, paleontology, agriculture, forensic medicine, among others.